NBRP Rat No: 0639 |
系統名: SD-Tg(Gfap-Tk)Jog |
通称: GFAP-TK |
Rat Genome Database |
研究責任者: |
Jonathan flint University of Oxford 〒 OX3 7BN Oxford Roosevelt Drive |
Tel: +44 (0)1865 287772 Fax: +44 (0)1865 287501 |
Email: jf@well.ox.ac.uk |
保存状況: |
胚 精子 生体 |
|
|
毛色 |
White |
近交世代数 |
More than 10 |
利用条件 |
利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 |
分類 |
|
コマーシャルブリーダー |
|
|
研究分野 |
|
遺伝子 |
Tk: thymidine kinase Gfap: glial fibrillary acidic protein |
由来 |
The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA).
The GFAP-TK rat was generated by pronuclear injection of a‘Gfap-Tk' Bacterial Artificial Chromosome (BAC) construct,
engineered using ‘RedET’-mediated homologous recombination methods (Groves et al., 2013). |
特性など |
Rats show no phenotype until dosed with the antiviral agant ganciclovir.
Chronic dosing at 10mg/kg/day via subcutaneous minipump for 21 days results in a loss of DCX-positive cells in the dentate gyrus and subventricular zone. |
繁殖・維持方法 |
Males are infertile. Females generate good litters of 10-15 pups showing 50% heterozygous offspring. |
遺伝診断法 |
GFAP-TK transgene can be identified using the foolwoing primers and protocols:
GFAP-TK_genoF cca acg gcg acc tgt ata ac
GFAP-TK_genoR gcc ctt tag tga ggg tta att c
Reagent Volume (ul)
Template DNA (10ng/ul) 2
Forward primer (10mMol) 1
Reverse Primer (10mMol) 1
Buffer (10x) 2
dNTP (8mMol) 2
Mg2+ (25mMol) 1.6
Taq Polymerase (Hot Star, Qiagen) 0.1
ddH20 10.3
Total Volume 20
Step Temp (°C) Time DNA activity
1 98 15 minutes Activation of polymerase
2 95 30s Denaturation
3 64 30s Annealing
4 -0.5 °C per cycle
5 72 1 min Extension
6 Go to step 3, 13 times
7 57 30s Annealing
9 72 1 min Extension
10 Got to step 7, 30 times
11 72 10 min final extension
12 4 forever |
参考文献 |
Groves JO, Leslie I, Huang GJ, McHugh SB, Taylor A, Mott R, Munafò M, Bannerman DM, Flint J.
Ablating Adult Neurogenesis in the Rat Has No Effect on Spatial Processing: Evidence from a Novel Pharmacogenetic Model.
PLoS Genet. 2013;9(9):e1003718. doi: 10.1371/journal.pgen.1003718. Epub 2013 Sep 5. |
系統情報 |
|
|