NBRP Rat No: 0739 |
系統名: F344-Depdc5em2kyo |
通称: F344-Depdc5em2Kyo/Kyo |
Rat Genome Database |
研究責任者: |
Stephanie BAULAC ICM - Institut du Cerveau et de la Moelle épinière 〒75013 Paris Hôpital Pitié-Salpêtrière 47 bd de l'Hôpital France |
Tel: +33 (0)1-5727-4339 Fax: +33 (0)1-5727-4339 |
Email: stephanie.baulac@upmc.fr |
保存状況: |
胚 精子 生体 |
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毛色 |
albino |
近交世代数 |
G2 (BCP2) |
利用条件 |
利用者は事前に寄託者の提供承諾書を得ること。 |
分類 |
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コマーシャルブリーダー |
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研究分野 |
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遺伝子 |
Depdc5: DEP domain containing 5 |
由来 |
This rat was created by the transcription activator-like effector nuclease (TALEN) technology. A pair of TALENs targeting exon 2 of rat Depdc5 (Ensembl: ENSRNOT00000085788) were microinjected into fertilized eggs of F344, and then transferred into the oviducts of pseudopregnant Wistar female rats, as previously described (Mashimo et al., 2013). TAL Effector Nucleotide Targeter 2.0 (https://tale-nt.cac.cornell.edu/) did not predict off-target site. |
特性など |
Homozygous Depdc5−/− embryos died from embryonic day 14.5 due to a global growth delay.
Heterozygous Depdc5+/− rats developed normally and exhibited no spontaneous electroclinical seizures, but had altered cortical neuron excitability and firing patterns. Depdc5+/− rats displayed cortical cytomegalic dysmorphic neurons and balloon-like cells strongly expressing phosphorylated rpS6, indicative of mTORC1 upregulation, and not observed after prenatal rapamycin treatment.
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繁殖・維持方法 |
Homozygous Depdc5−/− rats are lethal, but heterozygous Depdc5+/− rats are fertile. |
遺伝診断法 |
Primer (5'-3')
Forward AGCCTGACATTCTCGCTGTT
Reverse TCTTGCCCCACTCATTTACC
(product size 267bp)
Mutation c.39_55delinsT (p.Lys13Asnfs*8) (NM_001107229.1) |
参考文献 |
Marsan E, Ishida S, Schramm A, Weckhuysen S, Muraca G, Lecas S, Liang N, Treins C, Pende M, Roussel D, Le Van Quyen M, Mashimo T, Kaneko T, Yamamoto T, Sakuma T, Mahon S, Miles R, Leguern E, Charpier S, Baulac S.
Depdc5 knockout rat: A novel model of mTORopathy.
Neurobiol Dis. 2016 Feb 9. pii: S0969-9961(16)30031-6. |
系統情報 |
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