BLAST Help

Other option

Please input a parameter directly to perform a detailed setup. 

The option which can be specified is as follows. 
Please place "-" (minus) before an option and divide each parameter in a space. 
Examplej -G 2 -E 1 -q -2

G => Cost to open a gap [Integer] default=0  (zero invokes default behavior) 

E => Cost to extend a gap [Integer] default=0 (zero invokes default behavior) 

X => X dropoff value for gapped alignment (in bits) [Integer] default=0 (zero invokes default behavior) 

y => Dropoff (X) for blast extensions in bits (0.0 invokes default behavior) [Real] default = 0.0

I => Show GI's in deflines [T/F] default=F 

q => Penalty for a nucleotide mismatch (blastn only) [Integer] default=-3 

r => Reward for a nucleotide match (blastn only) [Integer] default=1 

v => Number of database sequences to show [Integer] default=500 

f => Threshold for extending hits, default if zero [Integer] default=0 

g => Perfom gapped alignment [T/F] (NA with tblastx) default=T 

D => DB Genetic code (for tblast[nx] only) default=1 

J => Believe the query defline [T/F] default=F 

W => Word size, default if zero [Integer] default=0 

z => Effective length of the database [Real] default=0 (use zero for the real size) 

n => MegaBlast search [T/F] default = F

A => Multiple Hits window size (zero for single hit algorithm) [Integer] default = 40

L => Location on query sequence [String]  Optional

Z => X dropoff value for final gapped alignment (in bits) [Integer] default = 0

U => Use lower case filtering of FASTA sequence [T/F] Optional default = F

S => Query strands to search against database (for blast[nx], and tblastx).  3 is both, 1 is top, 2 is bottom [Integer] default = 3

D => DB Genetic code (for tblast[nx] only) [Integer] default = 1

K => Number of best hits from a region to keep (off by default, if used a value of 100 is recommended) [Integer] default = 0

P => 0 for multiple hits 1-pass, 1 for single hit 1-pass, 2 for 2-pass [Integer] default = 0

Y => Effective length of the search space (use zero for the real size) [Real] default = 0

S => Query strands to search against database (for blast[nx], and tblastx) 3 is both, 1 is top, 2 is bottom [Integer] default = 3

A => Multiple Hits window size, default if zero (blastn/megablast 0, all others 40 [Integer] default = 0

t => Length of the largest intron allowed in tblastn for linking HSPs (0 disables linking) [Integer] default = 0



The following options can also input this ("Other options") column.
Priority is given to the value inputted into this column. 


Q => Query Genetic code to use [Integer] default=1 

e => Expectation vaule [Real] default=10.0 

F => Filter query sequence [String]  (DUST with blastn, SEG with others) default=T

w => Frame shift penalty (OOF algorithm for blastx) [Integer] default = 0
 
M => Matrix [String] default=BLOSUM62