系統情報
 NBRP Rat No: 0738  系統名: F344-Depdc5em1kyo  通称: F344-Depdc5em1Kyo/Kyo Rat Genome Database
研究責任者:  Stephanie BAULAC         ICM - Institut du Cerveau et de la Moelle épinière        〒75013  Paris Hôpital Pitié-Salpêtrière 47 bd de l'Hôpital       France
Tel: +33 (0)1-5727-4339    Fax: +33 (0)1-5727-4339 Email: stephanie.baulac@upmc.fr
保存状況:   胚            精子           生体
毛色  albino
近交世代数  G2 (BCP2)
利用条件  利用者は事前に寄託者の提供承諾書を得ること。
分類
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  その他 
コマーシャルブリーダー
研究分野
 糖尿病・肥満  脳神経疾患  眼疾患  歯科疾患  循環器系疾患・高血圧
 がん・腫瘍  代謝、内分泌  耳鼻疾患  免疫・アレルギー疾患  感染症
 骨形態異常  消化器疾患  皮膚疾患  生殖器疾患  発生
 行動・学習  血液疾患  泌尿器疾患  薬理学  その他  
 対照動物  レポーター遺伝子動物
遺伝子 Depdc5: DEP domain containing 5
由来 This rat was created by the transcription activator-like effector nuclease (TALEN) technology. A pair of TALENs targeting exon 2 of rat Depdc5 (Ensembl: ENSRNOT00000085788) were microinjected into fertilized eggs of F344, and then transferred into the oviducts of pseudopregnant Wistar female rats, as previously described (Mashimo et al., 2013). TAL Effector Nucleotide Targeter 2.0 (https://tale-nt.cac.cornell.edu/) did not predict off-target site.
特性など Homozygous Depdc5−/− embryos died from embryonic day 14.5 due to a global growth delay. Heterozygous Depdc5+/− rats developed normally and exhibited no spontaneous electroclinical seizures, but had altered cortical neuron excitability and firing patterns. Depdc5+/− rats displayed cortical cytomegalic dysmorphic neurons and balloon-like cells strongly expressing phosphorylated rpS6, indicative of mTORC1 upregulation, and not observed after prenatal rapamycin treatment.
繁殖・維持方法 Homozygous Depdc5−/− rats are lethal, but heterozygous Depdc5+/− rats are fertile.
遺伝診断法 Primer (5'-3') Forward AGCCTGACATTCTCGCTGTT Reverse TCTTGCCCCACTCATTTACC (product size 267bp) Mutation c.40_44delins17 (p.Gly15*) (NM_001107229.1)
参考文献 Marsan E, Ishida S, Schramm A, Weckhuysen S, Muraca G, Lecas S, Liang N, Treins C, Pende M, Roussel D, Le Van Quyen M, Mashimo T, Kaneko T, Yamamoto T, Sakuma T, Mahon S, Miles R, Leguern E, Charpier S, Baulac S.
Depdc5 knockout rat: A novel model of mTORopathy.
Neurobiol Dis. 2016 Feb 9. pii: S0969-9961(16)30031-6.
系統情報