Strain Name: Tg(zfRh1-3.7B:EGFP) / Allele: kj2 |
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| Allele | kj2 |
| Strain Name | Tg(zfRh1-3.7B:EGFP) |
| Type | Transgenics |
| Genotype | Tg(-3.7rho:EGFP)kj2 |
| Affected Gene | |
| Origin and Depositor | The University of Tokyo, Graduate School of Frontier Sciences, Department of Integrated Biosciences (Shoji Kawamura) |
| Link to ZFIN | ZDB-GENO-060830-2 |
| Information | |
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The reporter construct The 3.7-kb region immediately upstream of the zebrafish RH1 opsin (rod opsin or rhodopsin) was cloned into pEGFP-1(Clontech) plasmid at SalI/BamHI. Phenotype GFP fluorescence is observable in the retina after ~50 hpf (hours post fertilization) under a fluorescent microscope. It takes about 4~5 dpf (days post fertilization) for the GFP signal to be strong enough to be easily detectable under a stereo fluorescence microscope. Examination of transgene by PCR Genomic DNA is extracted from a fin clip at 1~2 months old. A portion of pEGFP sequence is PCR-amplified by the following primer pair: 5'-tacggcgtgcagtgcttcag-3' and 5'-tgttgtagttgtactccagc-3'. Details & Pictures |
| The Conditions to Distribute Strains | |
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In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hamaoka T, et al. (2002). Visualization of rod photoreceptor development using GFP-transgenic zebrafish. Genesis, 34 (3):215-220. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
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| Reference | |
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| Facility | RIKEN Center for Brain Science |