Strain Name: cfdp1 / Allele: ex2+130

Allele ex2+130
Strain Name cfdp1
Type Mutant
Genotype
Affected Gene cfdp1
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
A insertion-deletion mutation (+132 bp, -2bp) was introduced in the exon2 of cfdp1 gene by a CRISPR method. The mutation can be detected by PCR with the primers:
cfdp1-ex2+130-f, GATAACTTGAGTGAGGATGACA
cfdp1-ex2+130-r, CTCATATGGACATCTGCTTTAC

Mutation:
WT   GAAGGGGAGGATCA---------------------------------------------------------
---------------------------------------------------------------------------C
GACCGGCAGACGCCG
MUT  GAAGGGGAGGATCAGAAGGAAAAAATACTCATTTTTAACTATGGAAAATATTAGAAAGATTTTAACTAACA
GTATATAGAGAAATGAAGGTCAATGGAGTTGGAAATCTGCTACTTCATATTGTACTGCTCCCAAGAGTTGTAAAT-
-ACCGGCAGACGCCG
+132 bp, -2bp
					 
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Reference
 
Facility RIKEN Center for Brain Science