Strain Name: Tg(zfSWS1-5.5A:EGFP) / Allele: kj9 |
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| Allele | kj9 |
| Strain Name | Tg(zfSWS1-5.5A:EGFP) |
| Type | Transgenics |
| Genotype | Tg(-5.5opn1sw1:EGFP)kj9 |
| Affected Gene | |
| Origin and Depositor | The University of Tokyo, Graduate School of Frontier Sciences, Department of Integrated Biosciences (Shoji Kawamura) |
| Link to ZFIN | ZDB-GENO-080227-6 |
| Information | |
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The reporter construct The 5.5-kb region immediately upstream of the zebrafish SWS1 (UV-sensitive) opsin was cloned into pEGFP-1(Clontech) plasmid at EcoRI/SalI. Phenotype GFP fluorescence is observable in the retina after ~55 hpf (hours post fertilization) under a fluorescent microscope. It takes about 4~5 dpf (days post fertilization) for the GFP signal to be strong enough to be easily detectable under a stereo fluorescence microscope. Examination of transgene by PCR Genomic DNA is extracted from a fin clip at 1~2 months old. A portion of pEGFP sequence is PCR-amplified by the following primer pair: 5'-tacggcgtgcagtgcttcag-3' and 5'-tgttgtagttgtactccagc-3'. |
| The Conditions to Distribute Strains | |
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In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Takechi M, et al. (2003).Fluorescence visualization of ultraviolet-sensitive cone photoreceptor development in living zebrafish. FEBS Letters, 553 (1-2):90-94. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
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| Reference | |
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| Facility | RIKEN Center for Brain Science |