Home > 分離済み変異体 > tm246

分離済み変異体

Allele Name		tm246
Allele Type		バランス済
Sequence Name		C44C1.4a
Gene Name		vps-45
Worm Base	Allele Name	tm246
	Gene Name	vps-45
	Sequence	C44C1.4a
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype"		maternal effect larval lethal. Dr. G. Hermann: WT gut granules.Dr. S. L'Hernault: could not recover.Dr. S. Mitani: EMBOR 8, 152 (2007).
Mutation site Please see gene structure to locate the deletion in relation to exon(s)		15824/15825-17002/17003 (1178 bp deletion)
Chromosome		X
Putative gene structure		complement(join(12890..12941, 13131..13280, 13327..13449, 13801..13907, 13957..14049, 14571..14660, 14708..15203, 15774..15949, 16124..16188, 16236..16434, 16826..16918))
Map position		-18.69
Balancer		dpy-3 (e27) X
Map position of balancer		-15.81
Sequence of primers		IntRev:TCTCCTGCTCTACTTCTGCT,ExtFwd:ATGGGTGTACTGATCGTGAT,ExtRev:CTAGTACATCACGCATACGG,IntFwd:ATTTGCGTATGCACTGACCA
Distributed lab
Depositor		Dr. S. Mitani
References		Please submit your publication Los FC, Kao CY, Smitham J, McDonald KL, Ha C, Peixoto CA, Aroian RV. RAB-5- and RAB-11-dependent vesicle-trafficking pathways are required for plasma membrane repair after attack by bacterial pore-forming toxin. Cell Host Microbe 2011 9(2) 147-57  [ PubMed ID = 21320697 ] [ RRC reference ] Kage-Nakadai E, Kobuna H, Funatsu O, Otori M, Gengyo-Ando K, Yoshina S, Hori S, Mitani S. Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method. BMC Biotechnol 2012 12 1  [ PubMed ID = 22217006 ] [ RRC reference ] Maekawa M, Terasaka S, Mochizuki Y, Kawai K, Ikeda Y, Araki N, Skolnik EY, Taguchi T, Arai H. Sequential breakdown of 3-phosphorylated phosphoinositides is essential for the completion of macropinocytosis. Proc Natl Acad Sci U S A 2014 111(11) E978-87  [ PubMed ID = 24591580 ] [ RRC reference ] Kage-Nakadai E, Imae R, Yoshina S, Mitani S. Methods for single/low-copy integration by ultraviolet and trimethylpsoralen treatment in Caenorhabditis elegans. Methods 2014 68(3) 397-402  [ PubMed ID = 24613935 ] [ RRC reference ] Kage-Nakadai E, Imae R, Suehiro Y, Yoshina S, Hori S, Mitani S. A conditional knockout toolkit for Caenorhabditis elegans based on the Cre/loxP recombination. PLoS One 2014 9(12) e114680  [ PubMed ID = 25474529 ] [ RRC reference ] Delahaye JL, Foster OK, Vine A, Saxton DS, Curtin TP, Somhegyi H, Salesky R, Hermann GJ. Caenorhabditis elegans HOPS and CCZ-1 mediate trafficking to lysosome-related organelles independently of RAB-7 and SAND-1. Mol Biol Cell 2014 25(7) 1073-96  [ PubMed ID = 24501423 ] [ RRC reference ] Gengyo-Ando K, Kuroyanagi H, Kobayashi T, Murate M, Fujimoto K, Okabe S, Mitani S. The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans. EMBO Rep 2007 8(2) 152-7  [ PubMed ID = 17235359 ] [ RRC reference ] Hermann GJ, Schroeder LK, Hieb CA, Kershner AM, Rabbitts BM, Fonarev P, Grant BD, Priess JR. Genetic analysis of lysosomal trafficking in Caenorhabditis elegans. Mol Biol Cell 2005 16(7) 3273-88  [ PubMed ID = 15843430 ] [ RRC reference ]