Home   >   Zebrafish Transgenics List

>   Resource Information

Tabs(ALL, DNA, BLAST, GO, Taxonomy, DO, Reference) are clickable.

Resource count: 6,594,866

Resource Information Tg(zfRh1-3.7B:EGFP)

Resource Information
Organism	Zebrafish
strain or clone	Strain
Category	Transgenics
Resource name	Tg(zfRh1-3.7B:EGFP)
Availability	Available
Features	Allele : kj2 Information : The reporter constructThe 3.7-kb region immediately upstream of the zebrafish RH1 opsin (rod opsin or rhodopsin) was cloned into pEGFP-1(Clontech) plasmid at SalI/BamHI.PhenotypeGFP fluorescence is observable in the retina after ~50 hpf (hours post fertilization) under a fluorescent microscope. It takes about 4~5 dpf (days post fertilization) for the GFP signal to be strong enough to be easily detectable under a stereo fluorescence microscope.Examination of transgene by PCRGenomic DNA is extracted from a fin clip at 1~2 months old. A portion of pEGFP sequence is PCR-amplified by the following primer pair: 5'-tacggcgtgcagtgcttcag-3' and 5'-tgttgtagttgtactccagc-3'. Publications : Hamaoka T et al (2002) Genesis 34(3),215-20 Taxonomy : Siluriformes Reference : Noel NCL, Allison WT. (2018) Connectivity of cone photoreceptor telodendria in the zebrafish retina. J Comp Neurol 526(4) 609-625 Ojeda Naharros I, Cristian FB, Zang J, Gesemann M, Ingham PW, Neuhauss SCF, Bachmann-Gagescu R. (2018) The ciliopathy protein TALPID3/KIAA0586 acts upstream of Rab8 activation in zebrafish photoreceptor outer segment formation and maintenance. Sci Rep 8(1) 2211
Resouce detail information (request form)	http://shigen.nig.ac.jp/zebrafish/strainDetailAction.do?strainId=109

---

Distributors information
Organization	Developmental Gene Regulation. RIKEN Brain Science Institute
Person in charge	OKAMOTO Hitoshi
Postal Code
Address
Phone
FAX
E-mail	hitoshi.okamoto@riken.jp
URL
Other information

Page Top