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Resource Information KhES-2

Resource Information
Organism Embryonic stem cell
strain or clone Strain
Category Human ES cell
Resource name KhES-2
Availability Available
Features
Karyotype : normal diploid cell (female)
Stem Cell Marker : "Alkaline phosphatase, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, Nanog, Oct-3/4, Rex-1"
Differenciation potency : "neuronal cells and pigment cells were observed in in vitro culture, muscle, cartilage, gut-like epithelium were observed in teratomas."
Taxonomy Id : Homo sapiens
Reference : Hasegawa K, Fujioka T, Nakamura Y, Nakatsuji N, Suemori H. (2006) A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation. Stem Cells 24(12) 2649-60
Hattori F, Chen H, Yamashita H, Tohyama S, Satoh YS, Yuasa S, Li W, Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M, Makino S, Oikawa S, Fukuda K. (2010) Nongenetic method for purifying stem cell-derived cardiomyocytes. Nat. Methods 7(1) 61-6
International Stem Cell Initiative, Adewumi O, Aflatoonian B, Ahrlund-Richter L, Amit M, Andrews PW, Beighton G, Bello PA, Benvenisty N, Berry LS, Bevan S, Blum B, Brooking J, Chen KG, Choo AB, Churchill GA, Corbel M, Damjanov I, Draper JS, Dvorak P, Emanuelsson K, Fleck RA, Ford A, Gertow K, Gertsenstein M, Gokhale PJ, Hamilton RS, Hampl A, Healy LE, Hovatta O, Hyllner J, Imreh MP, Itskovitz-Eldor J, Jackson J, Johnson JL, Jones M, Kee K, King BL, Knowles BB, Lako M, Lebrin F, Mallon BS, Manning D, Mayshar Y, McKay RD, Michalska AE, Mikkola M, Mileikovsky M, Minger SL, Moore HD, Mummery CL, Nagy A, Nakatsuji N, O''Brien CM, Oh SK, Olsson C, Otonkoski T, Park KY, Passier R, Patel H, Patel M, Pedersen R, Pera MF, Piekarczyk MS, Pera RA, Reubinoff BE, Robins AJ, Rossant J, Rugg-Gunn P, Schulz TC, Semb H, Sherrer ES, Siemen H, Stacey GN, Stojkovic M, Suemori H, Szatkiewicz J, Turetsky T, Tuuri T, van den Brink S, Vintersten K, Vuoristo S, Ward D, Weaver TA, Young LA, Zhang W. (2007) Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nat Biotechnol 25(7) 803-16
Sumi T, Tsuneyoshi N, Nakatsuji N, Suemori H. (2007) Apoptosis and differentiation of human embryonic stem cells induced by sustained activation of c-Myc. Oncogene 26(38) 5564-76
Hasegawa K, Cowan AB, Nakatsuji N, Suemori H. (2007) Efficient multicistronic expression of a transgene in human embryonic stem cells. Stem Cells 25(7) 1707-12
International Stem Cell Initiative Consortium, Akopian V, Andrews PW, Beil S, Benvenisty N, Brehm J, Christie M, Ford A, Fox V, Gokhale PJ, Healy L, Holm F, Hovatta O, Knowles BB, Ludwig TE, McKay RD, Miyazaki T, Nakatsuji N, Oh SK, Pera MF, Rossant J, Stacey GN, Suemori H. (2010) Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells. In Vitro Cell. Dev. Biol. Anim. 46(3-4) 247-58
Takayama N, Nishikii H, Usui J, Tsukui H, Sawaguchi A, Hiroyama T, Eto K, Nakauchi H. (2008) Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors. Blood 111(11) 5298-306
Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N. (2006) Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage. Biochem Biophys Res Commun 345(3) 926-32
Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, Wataya T, Takahashi JB, Nishikawa S, Nishikawa S, Muguruma K, Sasai Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25(6) 681-6
Saeki K, Saeki K, Nakahara M, Matsuyama S, Nakamura N, Yogiashi Y, Yoneda A, Koyanagi M, Kondo Y, Yuo A. (2009) A feeder-free and efficient production of functional neutrophils from human embryonic stem cells. Stem Cells 27(1) 59-67
Resouce detail information
(request form)
http://shigen.nig.ac.jp/escell/human/top.jsp

Distributors information
Organization Stem Cell Reserch Center. Institute for Frontier Medical Sciences. Kyoto University
Person in charge
Postal Code
Address 53. Seigoin-Kawahara. Sakyoku. Kyoto-city
Phone 075-751-4646
FAX
E-mail esinfo@frontier.kyoto-u.ac.jp
URL http://www.frontier.kyoto-u.ac.jp/index.html
Other information