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Reference Related Resource Count :
33,482
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Reference Detail
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| Author |
Niklaus S, Cadetti L, Vom Berg-Maurer CM, Lehnherr A, Hotz AL, Forster IC, Gesemann M, Neuhauss SCF. |
| Reference |
Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters. |
| Journal |
eNeuro |
| Volume |
4(3) |
| Page |
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| Year Published |
2017 |
| Pubmed ID |
28612046
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Related Resource Count
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2 items
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| Organism |
strain |
Zebrafish
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Transgenics |
2 |
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| Organism |
DNA |
| Associated Resource is not found. |
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Category: Transgenics
| Allele : |
kj9 |
| Information : |
The reporter constructThe 5.5-kb region immediately upstream of the zebrafish SWS1 (UV-sensitive) opsin was cloned into pEGFP-1(Clontech) plasmid at EcoRI/SalI.PhenotypeGFP fluorescence is observable in the retina after ~55 hpf (hours post fertilization) under a fluorescent microscope. It takes about 4~5 dpf (days post fertilization) for the GFP signal to be strong enough to be easily detectable under a stereo fluorescence microscope.Examination of transgene by PCRGenomic DNA is extracted from a fin clip at 1~2 months old. A portion of pEGFP sequence is PCR-amplified by the following primer pair: 5'-tacggcgtgcagtgcttcag-3' and 5'-tgttgtagttgtactccagc-3'. |
| Publications : |
Takechi M et al (2003) FEBS Lett. 553(1-2),90-4 |
| Taxonomy : |
Siluriformes
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| Reference : |
Crespo C, Knust E. (2018) Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development. Biol Open 7(11)
D''Orazi FD, Zhao XF, Wong RO, Yoshimatsu T. (2016) Mismatch of Synaptic Patterns between Neurons Produced in Regeneration and during Development of the Vertebrate Retina. Curr Biol 26(17) 2268-79
Fang W, Guo C, Wei X. (2017) <i>Rainbow</i> Enhancers Regulate Restrictive Transcription in Teleost Green, Red, and Blue Cones. J Neurosci 37(11) 2834-2848
Nagashima M, Hadidjojo J, Barthel LK, Lubensky DK, Raymond PA. (2017) Anisotropic Müller glial scaffolding supports a multiplex lattice mosaic of photoreceptors in zebrafish retina. Neural Dev 12(1) 20
Niklaus S, Cadetti L, Vom Berg-Maurer CM, Lehnherr A, Hotz AL, Forster IC, Gesemann M, Neuhauss SCF. (2017) Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters. eNeuro 4(3)
Noel NCL, Allison WT. (2018) Connectivity of cone photoreceptor telodendria in the zebrafish retina. J Comp Neurol 526(4) 609-625
Sun C, Mitchell DM, Stenkamp DL. (2018) Isolation of photoreceptors from mature, developing, and regenerated zebrafish retinas, and of microglia/macrophages from regenerating zebrafish retinas. Exp Eye Res 177 130-144
Zimmermann MJY, Nevala NE, Yoshimatsu T, Osorio D, Nilsson DE, Berens P, Baden T. (2018) Zebrafish Differentially Process Color across Visual Space to Match Natural Scenes. Curr Biol 28(13) 2018-2032.e5
Li YN, Tsujimura T, Kawamura S, Dowling JE. (2012) Bipolar cell-photoreceptor connectivity in the zebrafish (Danio rerio) retina. J Comp Neurol 520(16) 3786-802
Novales Flamarique I. (2016) Diminished foraging performance of a mutant zebrafish with reduced population of ultraviolet cones. Proc Biol Sci 283(1826) 20160058
Yoshimatsu T, D''Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, Raible DW, Wong RO. (2016) Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo. Nat Commun 7 10590
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Category: Transgenics
| Allele : |
kj11 / tkk008 |
| Information : |
Zebrafish have one type of rod and four types of cone photoreceptor cells in the retina. The four cone types express phylogenetically and spectrally distinct types of visual opsins. Using zebrafish as a model animal, we are exploring the molecular mechanism of the cell-type specific expression of the opsin genes. We make DNA constructs where test genomic regions in or around the opsin genes are conjugated to the GFP (Green Fluorescent Protein)-related reporter genes and introduce them to zebrafish embryos to establish transgenic lines. The Tg(zfSWS2-3.5A:EGFP)tkk008 is one of such transgenic zebrafish lines, where the 3.5-kb region immediately upstream of the SWS2 opsin (blue type opsin) gene is introduced with the EGFP reporter. It expresses GFP specifically in the long single cones (LSCs) of the retina. In effect, LSCs of the zebrafish are visualized by GFP. The fish would be useful for the studies of retinal development and screening of retinal mutants. |
| Publications : |
Takechi M et al (2008) J. Biol. Chem. 283(46),31625-32 |
| Taxonomy : |
Siluriformes
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| Reference : |
D''Orazi FD, Zhao XF, Wong RO, Yoshimatsu T. (2016) Mismatch of Synaptic Patterns between Neurons Produced in Regeneration and during Development of the Vertebrate Retina. Curr Biol 26(17) 2268-79
Niklaus S, Cadetti L, Vom Berg-Maurer CM, Lehnherr A, Hotz AL, Forster IC, Gesemann M, Neuhauss SCF. (2017) Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters. eNeuro 4(3)
Li YN, Tsujimura T, Kawamura S, Dowling JE. (2012) Bipolar cell-photoreceptor connectivity in the zebrafish (Danio rerio) retina. J Comp Neurol 520(16) 3786-802
Yoshimatsu T, D''Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, Raible DW, Wong RO. (2016) Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo. Nat Commun 7 10590
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