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Resource count: 6,595,109
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Resource Information
Tg(zfSWS2-3.5A:EGFP)
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| Organism |
Zebrafish |
| strain or clone |
Strain |
| Category |
Transgenics |
| Resource name |
Tg(zfSWS2-3.5A:EGFP) |
| Availability |
Available
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| Features |
| Allele : |
kj11 / tkk008 |
| Information : |
Zebrafish have one type of rod and four types of cone photoreceptor cells in the retina. The four cone types express phylogenetically and spectrally distinct types of visual opsins. Using zebrafish as a model animal, we are exploring the molecular mechanism of the cell-type specific expression of the opsin genes. We make DNA constructs where test genomic regions in or around the opsin genes are conjugated to the GFP (Green Fluorescent Protein)-related reporter genes and introduce them to zebrafish embryos to establish transgenic lines. The Tg(zfSWS2-3.5A:EGFP)tkk008 is one of such transgenic zebrafish lines, where the 3.5-kb region immediately upstream of the SWS2 opsin (blue type opsin) gene is introduced with the EGFP reporter. It expresses GFP specifically in the long single cones (LSCs) of the retina. In effect, LSCs of the zebrafish are visualized by GFP. The fish would be useful for the studies of retinal development and screening of retinal mutants. |
| Publications : |
Takechi M et al (2008) J. Biol. Chem. 283(46),31625-32 |
| Taxonomy : |
Siluriformes
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| Reference : |
D''Orazi FD, Zhao XF, Wong RO, Yoshimatsu T. (2016) Mismatch of Synaptic Patterns between Neurons Produced in Regeneration and during Development of the Vertebrate Retina. Curr Biol 26(17) 2268-79
Niklaus S, Cadetti L, Vom Berg-Maurer CM, Lehnherr A, Hotz AL, Forster IC, Gesemann M, Neuhauss SCF. (2017) Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters. eNeuro 4(3)
Li YN, Tsujimura T, Kawamura S, Dowling JE. (2012) Bipolar cell-photoreceptor connectivity in the zebrafish (Danio rerio) retina. J Comp Neurol 520(16) 3786-802
Yoshimatsu T, D''Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, Raible DW, Wong RO. (2016) Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo. Nat Commun 7 10590
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Resouce detail information
(request form)
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http://shigen.nig.ac.jp/zebrafish/strainDetailAction.do?strainId=149
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| Organization |
Developmental Gene Regulation. RIKEN Brain Science Institute |
| Person in charge |
OKAMOTO Hitoshi |
| Postal Code |
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| Address |
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| Phone |
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| FAX |
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| E-mail |
hitoshi.okamoto@riken.jp
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| URL |
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| Other information |
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